Sphingosine Kinase 2 Inhibitor Accelerates Fas-Mediated Cell Death Progression in A20/2J Cells
Miki Hara-Yokoyama*, 1, Kazue Terasawa1, Akio Kihara2, Jin-Wook Kim2, 3, Chan-Seo Park2, 4, Yoshio Hirabayashi5, Yasuyuki Igarashi2 , Masaki Yanagishita1
1 Department of Hard Tissue Engineering, Biochemistry, Division of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan,
2 Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-choume, Kita-ku, Sapporo 060-0812, Japan,
3 LCS biotech, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea, (Present address)
4 Department of Chemical and Biochemical Engineering, Dongguk University, 3-26, Pil-dong, Chung-gu, Seoul 100-715, Korea (Present address)
5 Brain Science Institute, The Institute of Physical and Chemical Research (Riken), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Abstract
A sphingosine kinase (SPHK) type 2 inhibitor, SG-12, inhibited the SPHK2 activity in A20/2J cells and promoted Fas-dependent cell death. The effect of SG-12 on cell death was relieved by SPHK2 transfection but not by SPHK1 transfection. The results suggest a SPHK2-specific suppression of Fas-dependent cell death.
Key Words: Sphingosine-1-phosphate, Sphingosine kinase 2, Fas, Cell death, B cell.
Article Information
Article History:
Received Date: 7/8/2008
Revision Received Date: 16/9/2008
Acceptance Date: 19/9/2008
Electronic publication date: 28/11/2008
Collection year: 2008
© Ahlberg etal.; Licensee Bentham Open.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (
http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the Department of Hard Tissue Engineering, Biochemistry, Division of Bio-Matrix, Graduate School Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo, Tokyo 113-8549, Japan; Tel: +81 3 5803 5567; Fax: +81 3 5803 0187;
E-mail: m.yokoyama.bch@tmd.ac.jp
Open Peer Review Details |
Manuscript submitted on 7-8-2008 |
Original Manuscript |
Sphingosine Kinase 2 Inhibitor Accelerates Fas-Mediated Cell Death Progression in A20/2J Cells
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