RESEARCH ARTICLE
High-Yield Laccase-Producing Strains Constructed by Protoplast Fusion Between Bacterium and Fungus
Lihong Zhao*, Wenli Yin, Lele Wang
Article Information
Identifiers and Pagination:
Year: 2015Volume: 9
First Page: 221
Last Page: 224
Publisher ID: TOBIOTJ-9-221
DOI: 10.2174/1874070701509010221
Article History:
Received Date: 17/02/2015Revision Received Date: 21/03/2015
Acceptance Date: 09/06/2015
Electronic publication date: 2/11/2015
Collection year: 2015
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
This study is based on the construction of high-yield laccase-producing fusant achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions were demonstrated with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened for different characteristics between two parental strains and further identified by laccase activity, offering one of the genetically stable fusants, Strain F. The fusant F produced the highest yield of laccase, being about 22% higher than that of the parental strain. The results suggest that the protoplast fusion technique can be considered as a promising technique for the control of environmental pollution.