RESEARCH ARTICLE


High-Yield Laccase-Producing Strains Constructed by Protoplast Fusion Between Bacterium and Fungus



Lihong Zhao*, Wenli Yin, Lele Wang
Civil & Architectural Engineering College, Liaoning University of Technology, Jinzhou, China


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Creative Commons License
© 2015 Zhao et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Civil & Architectural Engineering College, Liaoning University of Technology, Jinzhou, China; Tel: +18904162944; E-mail: zhaolh05@163.com; 254297630@qq.com


Abstract

This study is based on the construction of high-yield laccase-producing fusant achieved by inter-kingdom protoplast fusion between Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditions were demonstrated with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants were screened for different characteristics between two parental strains and further identified by laccase activity, offering one of the genetically stable fusants, Strain F. The fusant F produced the highest yield of laccase, being about 22% higher than that of the parental strain. The results suggest that the protoplast fusion technique can be considered as a promising technique for the control of environmental pollution.

Keywords: Escherichia coli, Laccase, Pleurotus ostreatus, Protoplast fusion, White rot fungi.