High Glucose Enhances Skin Sensitizer-induced NRF2 Activation In Vitro
Takeo Takeda1, Masahiro Ogawa1, *, Junya Kitamoto1, Takahiro Kyoya1, Megumi Terada1
1 Life Science Research Institute, Kumiai Chemical Industry Co. Ltd., Shizuoka 439-0031, Japan
Abstract
Background:
Hyperglycemia has a potentially critical role in the promotion of sensitization, however, the clear mechanism of this phenomenon is unknown. The activation of NRF2 is a key event triggered by skin sensitizers. Therefore, we investigated the effects of high glucose on the activation of NRF2 by the skin sensitizers in vitro.
Methods:
The involvement of glucose levels in NRF2 activation by cinnamaldehyde, a skin sensitizer, was assessed in human MCF-7 breast cancer cells under normal glucose conditions (1.0 g/L D-glucose) and high glucose conditions (4.5 g/L D-glucose).
Results:
High glucose induced the NRF2 transactivation, HMOX1 mRNA expression, and SOD-like activity. Nuclear NRF2 level was increased under high glucose conditions compared to normal glucose conditions. High glucose also enhanced the cinnamaldehyde-induced HMOX1 mRNA expression and SOD-like activity.
Conclusion:
Oxidative stress caused by hyperglycemia induced additionally the activation of NRF2 signaling by skin sensitizers.
Keywords: Glucose, Skin sensitizer, Cinnamaldehyde, Oxidative stress, KEAP1-NRF2 signaling, ROS.
Article Information
Article History:
Received Date: 22/10/2020
Revision Received Date: 3/3/2021
Acceptance Date: 12/3/2021
Electronic publication date: 19/4/2021
Collection year: 2021
© 2021 Takeda et al
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at:
https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
* Address correspondence to this author at the Life Science Research Institute, Kumiai Chemical Industry Co. Ltd., Shizuoka, Japan; Tel: +81-537-35-3160; Fax: +81-537-36-3718; E-mail: m-ogawa@kumiai-chem.co.jp
Open Peer Review Details |
Manuscript submitted on 22-10-2020 |
Original Manuscript |
High Glucose Enhances Skin Sensitizer-induced NRF2 Activation In Vitro |