Fig. (3). Surefire assay principle The AlphaScreen platform can be used to detect analytes in a crude cell lysate using protocols developed into the Surefire format. Here, the levels of a phosphorylated protein (such as phosphoERK_1/2 can be quantified using the approach, by use of specific antibodies to the phosphoprotein, without wash and separation steps as in a typical ELISA assay.