| ELISA | AlphaLISA | |
|---|---|---|
| Selectivity | High | High |
| Sensitivity | Sensitive (pM range) | Sensitive (sub-pM) |
| Assay Format | Heterogeneous | Homogenous |
| Antibody req. | Requires matched antibody pairs | Used with antibody pairs |
| Assay vol. | large (25-50 µl) | small (≥ 5 µl) |
| Dynamic range | limited (~2 orders of mag.) | large (~4 orders of mag.) |
| Instrumentation | any luminescent reader | requires high energy laser |