| AlphaScreen/AlphaLISA™ | LANCE | DELFIA | |
|---|---|---|---|
| Detection | Luminescence Proximity | TR-FRET | TRF |
| Homogenous | Yes | Yes | No |
| Throughput | High | Ultra-High | Medium |
| Automation | **** | **** | * |
| Sensitivity | **** | *** | **** |
| Dynamic range | 2.5 - 5 logs | 2 - 3 logs | 2.5 - 5 logs |
| Microplate formats | 96, 384, 1536 | 96, 384, 1536 | 96, 384 |
| Multiplexing | No | No | up to 4-plex |
| Substrate sizes | Small molecules to whole cells | Small molecules to peptides | Small molecules to proteins, |
| Use of low affinity antibodies | Yes | Limited; impact on data quality | No |
| Use of polyclonal antibodies | Yes | Limited; only special affinity purified Abs | Yes |
| Reader | EnVision | EnVision, Victor, ViewLux & others | EnVision, Victor, ViewLux & others |