Fig. (2) The numbers of recovered MDCK cells coated with 1% agarose. 0.4×106 MDCK cells were seeded in 60 mm dish to grow for 24 hours at 37 °C, 5% CO2 incubator, and then MDCK cells were coated with 1% agarose-medium and mimic shipping condition for 1 to 7 days. The recovered cells were counted after removing the agarose-medium gel and a 24 hours incubation in the regular cell culture condition.