Fig. (2) MMTV promoter is activated by multiple full length receptors. A) HEK293 were transiently transfected with pGL4.36[MMTV/luc2P/hygro] or the MMTV promoter driving luc2 and vector containing a CMV driven full length AR. For a negative control, pGL4.36[MMTV/luc2P/hygro] was co-transfected with pUC19 vector. Cells were then induced with 1:5 dilutions of Dihydrotestosterone (DHT). B) HeLa cells were transiently transfected with pGL4.36[MMTV/luc2P/hygro] in 96-well plates, then induced with 1:4 serial dilutions of hydrocortisone or dexamethasone.