Fig. (5) Dose response to wortmannin and HC in Clone 10 cells. Each point represents Mean ± SD for 6 replicates. HC inhibits the lysosomal degradation of the autophagosomes so that more accumulate for imaging with increasing doses of HC. Wortmannin IC₅₀ was similar at 5 and 10 μM HC (1.4 nM). A dose of 33 nM for wortmannin and 10 μM for HC was selected to produce a good signal: background and best Z’.