Polymorphic Site |
PCR Primer |
Position (*) |
SNP |
SBE Primer |
Position (*) |
7025 |
5´-CTGACTGGCATTGTATTAGCA-3´ |
6960F |
C7028T |
5´-ACACGACACGTACTACGTTGTAGC-3´ |
7004F |
5´-GTATACGGGTTCTTCGAATG-3´ |
7433R |
14766 |
5´-GAGAAGGCTTAGAAGAAAACCCCAC-3´ |
14601F |
T14766C |
5'-cgatcATGAGTGGTTAATTAATTTTATTA-GGGGGTTA-3' |
14798R |
5´-GTGGGCGATTGATGAAAAGGC-3´ |
14950R |
10394 |
5´-GGCCTATGAGTGACTACAAAAA-3´ |
10364F |
A10398G |
5'-ataTATGAGTGACTACAAAAAGGATTAGACTGA-3' |
10368F |
5´-TATTCCTAGAAGTGAGATGGT-3´ |
10526R |
4577 |
5´-CCTACCACTCACCCTAGCATTAC-3´ |
4185F |
G4580A |
5'-(at)7TTTTTTACCTGAGTAGGCCTAGAAATAAACAT-3' |
4548F |
5´-TAGGAATGCGGTAGTAGTTAG-3´ |
5120R |
12308 |
5´-CAACCCCGACATCATTACCGGGT-3´ |
12106F |
A12308G |
5'-(tacg)5aCCATTGGTCTTAGGCCCCAA-3' |
12288F |
5´-GGGTTAACGAGGGTGGTAAGG-3´ |
12413R |
4216 |
5´-CCTACCACTCACCCTAGCATTAC-3´ |
4185F |
T4216C |
5´-cgCCACTCACCCTAGCATTACTTATATGA-3´ |
4189F |
5´-GCGAGCTTAGCGCTGTGATGAG-3´ |
4542R |
10032(**) |
5´-CTTTGGCTTCGAAGCCGCCGCC-3´ |
9902F |
A10029G |
|
|
5´-TATTCCTAGAAGTGAGATGGT-3´ |
10526R |
|
|
14465 |
5´-ATGCCTCAGGATACTCCTCAATAGCCATC-3´ |
14430F |
T14470C |
|
|
5´-CCGTGCGAGAATAATGATGTATGC-3´ |
14686R |
|
|
8994(**) |
5´-TAGCCCACTTCTTACCACAAGGC-3´ |
8900F |
G8994A |
|
|
5´-GTGTGAAAACGTAGGCTTG-3´ |
9172R |
|
|
Lower case letters indicate the unspecific nucleotides in 5´-end of the SBE primer.
(*) R: primer in reverse orientation; F: primer in forward orientation.
(**) Digested PCR products will appear like three fragments in an agarose gel, whereas undigested products like two fragments.