Fig. (4) Immunoprecipitation of dysferlin-interacting partners with
anti-dysferlin. The levels of the indicated proteins in the lysates
before immunoprecipitation were detected by Western blot analysis
(lane 1). To confirm interactions detected by the TAP tag technique,
immunoprecipitations were conducted with a dysferlinspecific
antibody (lane 2). As control, no dysferlin-specific antibody
was added to the lysates (lane 3).