Cells from one CLL patient were cultured at 2 × 106/ml in the presence or not of
Resiquimod 1µM, together or not with BAFF-R-Fc (50µg/ml) or BCMA-Fc (50
µg/ml). After 48 hours of incubation, 20 000 cells were collected and the percentage of
enrichment in cytoplasmic nucleosomes was estimated from triplicate samples by a
specific ELISA, as described in Materials and Methods. Statistical significances in
comparison with control (unstimulated) cells, taken as 100%, were estimated with the
modified Student’s t test for a small number of samples. Data are from one patient out
of three that gave comparable results.