Table 1: Specification of the domain biology.

DOMAIN BIOLOGY
INDICATOR BIODETERIORATION
PARAMETER
  1. Total number of cultivable organisms (autotrophic, heterotrophic, chemio-synthetic)
  2. Morphological and functional identification (number of genus - species)
  3. Molecular Identification (taxonomic categories)
  4. Fatty acids
  5. N/C Report
  6. Micro fauna and weeds (type and specification)
  7. Air contamination in the area
  8. Substrate Type
INSTRUMENT
  1. Techniques of Environmental Microbiology
  2. Optical microscopy SEM and CLSM, Biolog, API
  3. DGGE, real-Time PCR, FISH
  4. Gas Chromatograph
  5. Luminometer
  6. Elemental Analysis
  7. Sampling and direct observation (MO)
  8. Aerobiology
  9. XRD - XRF, Thin section
PROTOCOL
  1. Sample type (tool, quantity, transport), Treatment type (suspension-dilution), Type of medium (total microflora, actinomycetes, fungi, algae, protozoa, nitrogen cycle, carbon, sulfur) , Type of Inoculation, incubation and counts (CFU / MPN)
  2. Macroscopic and microscopic observation of isolated Physiological and Biochemical test
  3. Extraction and cloning of DNA, with PCR Real Time, FISH, T-RFLP
  4. DGGE analysis
  5. Extraction and determination of the ATP
  6. Chemical analysis for nitrogen and organic carbon (TOC)
  7. Type of sampling classification and counting
  8. Device and methods used
  9. Composition and structure of the substrate (mineralogical and petro graphic analyses)