Probeb
|
Clone 1 > 18fc Nucleotide Change |
18fc Nucleotide Position |
Number |
Sequence |
Nucleotide Range |
1 |
GTTATTTATATGGCAAAAGAGGGGGAGGA |
4394 - 4422 |
T > C |
4402 |
38 |
ACAGATGAGGATTGGTCAGATTTTTGTCA |
4579 - 4607 |
T > C |
4590 |
109 |
TTGAAATTAGAAAACAAAACATGACTGAA |
4934 - 4962 |
A > T |
4938 |
120d
|
TTCAGATGATGATAATGATAGTGCAGTAG |
4989 - 5014 |
GAT > del |
4998/4999 |
152 |
GGATGGTTTGTGTATAAGCATTTCTCCCG |
5146 - 5174 |
T > G |
5152 |
154 |
TGTATAAGCATTTCTCCCGCAAAGAGGAA |
5156 - 5184 |
G > A |
5174 |
235 |
GAGATATTACTCAGCATTTTATTAAGAAA |
5561 - 5589 |
C > G |
5572 |
409 |
ATGAATTGAGACCATTAGAGAAAGTGTTG |
6431 - 5559 |
A > G |
6441 |
441 |
TCCTGATAGACAGTGGGATGAATTATTTA |
6591 - 6619 |
G > A |
6599 |
443e
|
CAGTGGGATGAATTATTTAAAACAATGAT |
6601 - 6629 |
T > C |
6613 |
501 |
TGTGTTTTCCAAGATATTTGGAAAGTCTC |
6891 - 6919 |
C > T |
6900 |
a Detection of (putative) nucleotide differences by array hybridization between clone 1 and 18f was initially based on the hybridization profile in Fig. (
6).
b Probe numbers are from Fig. (
6) and represent the oligonucleotide probes whose sequence contains nucleotide change(s) between clone 1 and 18f when the clone 1 target sequence is identical to the probe sequence. Nucleotide changes were indentified (grey boxed) based on comparison of the clone 1 and 18f GenBank sequences (accession numbers in Fig. (
1)) used to develop the HAV1Cb group 1 consensus probe set (Fig.
1). The probe nucleotide range numbering is defined by the 29-mer probe and corrected to 18f nucleotide numbering from Lemon
et al. [
21].
c The nucleotide change and position between clone 1 and 18f as reported by Lemon
et al. [
21].
d Probe 120 defines a 26 nucleotide base region of 18f due to the three-base GAT deletion.
e This probe identified as a potential peak overlapping with the peak at probe 441.