Fig. (1) CypI prevent IFN-induced PKR activation in HCV-infected cells. (A) Schematic diagram of genomic JFH-1 replicon used is depicted. JFH-1-infected Huh7.5.1 cells were treated with or without the CypI alisporivir (ALV) or NIM811, and subsequently treated with IFNα or β for 24 or 48 h. Cells were then lysed and analyzed for their content in viral and host proteins by Western blotting. (B) Schematic diagram of subgenomic JFH-1 replicon used is depicted. Same as (A) except that JFH-1 subgenomic replicon cells were used and treated with IFNα for 24 h. (C) Same as (A) except that JFH-1-infected Huh7.5.1 cells were treated with a panel of CypI as well as with or without IFNα for 24 h. Results are representative of 3-5 independent experiments.