RESEARCH ARTICLE
Inhibition of Mycelial Growth of Rhizoctonia Solani by Chitosan in vitro and in vivo
Sabah R. Mohammed1, 2, *, Elsayed M. Zeitar3, Ivan D. Eskov1
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
First Page: 156
Last Page: 161
Publisher ID: TOASJ-13-156
DOI: 10.2174/1874331501913010156
Article History:
Received Date: 18/07/2019Revision Received Date: 20/09/2019
Acceptance Date: 13/10/2019
Electronic publication date: 20/12/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective:
Evaluate the antifungal effect of chitosan against Rhizoctonia solani in vitro and the possible mechanisms of its induced activity in potato tubers to control black scurf disease.
Methods:
The in vitro influence of chitosan at different concentrations on mycelial growth of R. solani was tested by using the poisoned food technique in PDA medium. The effect of these concentrations on the development of lesion diameters in tubers inoculated with R. solani mycelium was assayed for 30 days. The concentration that showed the greatest inhibitory effect on lesion diameters was tested to assess the induced activity of defense-related enzymes in the infected tubers.
Results:
In the poisoned food technique, chitosan at 1% completely inhibited the growth of R. solani mycelium. In vivo tests showed that chitosan treatment at 0.5% effectively controlled the black scurf in tubers inoculated with R. solani mycelium. Chitosan increased the activities of defense-related enzymes such as Peroxidase (POD), Polyphenol Oxidase (PPO) and Phenylalanine Ammonia-lyase (PAL) in treated tubers of tested cultivars.
Conclusion:
This work demonstrated that chitosan directly inhibited the growth of R. solani, and potentially elicited defense reaction in potato tubers.