Analysis of Raspberry Ketone in Nutraceutical Formulation Using Fourier Transform Infrared Spectrophotometric Method
Hassan Y. Aboul-Enein1, *, Oana Mihaela Antochi2, 3, Gheorghe Nechifor3, Andrei A. Bunaciu4
1 Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Dokki, Cairo 12622, Egypt
2 SCIENT – Research Center for Instrumental Analysis, 1 Petre Ispirescu Street, Tancabesti - Snagov, 077617, Romania
3 Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica Bucharest, 1-7 Polizu Street, Bucharest - 1, 011061, Romania
4 AAB_IResearch Laboratory, 7 Modoran Ene Street, Bucharest – 5, 051832, Romania
A Fourier Transform Infrared (FT-IR) spectrometric method was developed for the rapid, direct measurement of Raspberry Ketone (RK) and Caffeine (CAF) in a nutraceutical formulation.
Conventional KBr-spectra and KBr+0.5 mg Microcrystalline Cellulose (MCC)-spectra were used as the basis for a better determination of active substances in the nutraceutical formulation. A calibration model was developed using caffeine and raspberry ketone standards of varying concentrations in the mid-infrared region (4000-400 cm-1). The Beer-Lambert law was used in data processing.
The results indicate that FT-IR spectrometry is applicable to the analytical quantification of RK and CAF in the nutraceutical formulation.
The method proposed is simple, precise and not time-consuming compared to the chromatographic methods that are cited in the literature. Quantification is performed in about 10-15 minutes, including sample preparation and spectral acquisition.
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* Address correspondence to this author at the Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Dokki, Cairo 12622, Egypt; Tel: +00201003678948; Fax: +0020233370931;