The Open Bioactive Compounds Journal


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Analysis of Raspberry Ketone in Nutraceutical Formulation Using Fourier Transform Infrared Spectrophotometric Method

Hassan Y. Aboul-Enein1, *, Oana Mihaela Antochi2, 3, Gheorghe Nechifor3, Andrei A. Bunaciu4
1 Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Dokki, Cairo 12622, Egypt
2 SCIENT – Research Center for Instrumental Analysis, 1 Petre Ispirescu Street, Tancabesti - Snagov, 077617, Romania
3 Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica Bucharest, 1-7 Polizu Street, Bucharest - 1, 011061, Romania
4 AAB_IResearch Laboratory, 7 Modoran Ene Street, Bucharest – 5, 051832, Romania



A Fourier Transform Infrared (FT-IR) spectrometric method was developed for the rapid, direct measurement of Raspberry Ketone (RK) and Caffeine (CAF) in a nutraceutical formulation.


Conventional KBr-spectra and KBr+0.5 mg Microcrystalline Cellulose (MCC)-spectra were used as the basis for a better determination of active substances in the nutraceutical formulation. A calibration model was developed using caffeine and raspberry ketone standards of varying concentrations in the mid-infrared region (4000-400 cm-1). The Beer-Lambert law was used in data processing.


The results indicate that FT-IR spectrometry is applicable to the analytical quantification of RK and CAF in the nutraceutical formulation.


The method proposed is simple, precise and not time-consuming compared to the chromatographic methods that are cited in the literature. Quantification is performed in about 10-15 minutes, including sample preparation and spectral acquisition.

Keywords: Microcrystalline cellulose, Data processing, Ketone standards, Spectrometric method, Nutraceutical formulation, Analytical quantification.

Article Information

Identifiers and Pagination:

Year: 2019
Volume: 7
First Page: 8
Last Page: 13
Publisher Id: TOBCJ-7-8
DOI: 10.2174/1874847301907010008

Article History:

Received Date: 09/07/2019
Revision Received Date: 27/09/2019
Acceptance Date: 17/10/2019
Electronic publication date: 05/12/2019
Collection year: 2019

© 2019 Aboul-Enein et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, Dokki, Cairo 12622, Egypt; Tel: +00201003678948; Fax: +0020233370931;

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