RESEARCH ARTICLE


Characterization of Liposomes for Cancer Cell Transfection



Svetlana A Tatarkova*, 1, Satvinder Khaira2
1 Department of Physics, Durham University, South Road, Durham, DH1 3LE, UK
2 University of Manchester, Manchester, M13 9PL, UK


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Creative Commons License
2007 Bentham Science Publishers Ltd.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/) which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

* Address correspondence to this author at the Department of Physics, Durham University, South Road, Durham, DH1 3LE, UK; E-mail: s.a.tatarkova@durham.ac.uk


Abstract

We have characterized a broad range of liposome formulations with varying DcChol:DOPE ratio. Subsequent addition of DcChol to liposomes increases its positive surface charge. However, loading the nuclear acids did not neutralize the overall negative surface potential to a similar extent. The liposomes were tested by transfection of DNA in living cancer cells.