Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor
Christian Weber1, Sebastian Pohl1, Ralf Pörtner2, Christine Wallrapp3, Moustapha Kassem4, Peter Geigle3, Peter Czermak*, 1, 5
1 Institute of Biopharmaceutical Technology, University of Applied Sciences Giessen-Friedberg, Giessen-Germany
2 Institute of Bioprocess and Biosystems Engineering, University of Technology, Hamburg-Germany
3 CellMed AG, Alzenau, Germany
4 Department of Endocrinology and Metabolism, University Hospital of Odense, Odense-Denmark
5 Department of Chemical Engineering, Kansas State University, Manhattan KS-USA
The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive oxygen sensor for the continuous monitoring of the cultivation process. Fixed bed bioreactors offer advantages in comparison to other systems due to their ease of automation and online monitoring capability during the cultivation process. These benefits combined with the advantage of single-use make the fixed bed reactor an interesting option for GMP processes. The cultivation of the encapsulated cells in the fixed bed bioreactor system offered vitalities and adipogenic differentiation similar to well-mixed suspension cultures.
Received Date: 1/8/2007 Revision Received Date: 12/10/2007 Acceptance Date: 12/10/2007 Electronic publication date: 29/10/2007 Collection year: 2007
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2007 Bentham Science Publishers Ltd.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
* Address correspondence to this author at the Institute of Biopharmaceutical Technology, University of Applied Sciences Giessen-Friedberg, Wiesenstrasse 14, 35390 Giessen, Germany; E-mail: Peter.Czermak@tg.fh-giessen.de