The Open Biomedical Engineering Journal

ISSN: 1874-1207 ― Volume 13, 2019

Characterization of the F-box Proteins FBXW2 and FBXL14 in the Initiation of Bone Regeneration in Transplants given to Nude Mice

Mari Akiyama*
Department of Biomaterials, Osaka Dental University, Osaka 573-1121, Japan



Cultured bovine-periosteum-derived cells can form three-dimensional structures on tissue culture dishes without artificial scaffolding material, can induce bone regeneration in vivo. The utility of cultured bovine-periosteum-derived cells for bone tissue regeneration after their transplantation into nude mice has been reported, the precise F-box molecular mechanism was unclear.


The aim of this study was to investigate the specific F-box proteins required for bone regeneration by cultured bovine-periosteum-derived cells in vitro.


In the present study, periosteum tissue and cultured periosteum-derived cells were cultured for 5 weeks in vitro and then embedded in collagen gel with a green tissue-marking dye. Electrophoresis and immunohistochemistry were used to identify the specific F-box proteins required for tissue bone regeneration.


The bovine-periosteum-derived cells were observed to form bone shortly after the expression of F-box proteins. After the initial phase of bone formation, the expression of the F-box proteins ceased. FBXW2 was shown to be expressed in the periosteum, but not in cultured periosteum-derived cells. Furthermore, FBXL14 disappeared during bone formation.


Bone regeneration requires progenitor cells, such as bovine-periosteum-derived cells and the activation of the F-box Proteins FBXW2 and FBXL14, over time the expression of these proteins ceases. Further scientific and clinical trials are needed to investigate how the F-box Proteins can be used therapeutically to treat osteoporosis and osteonecrosis.

Keywords: Cultured bovine-periosteum-derived cells, FBXW2, FBXL14, Bone regeneration, Osteocalcin, TRAP staining.

Article Information

Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 75
Last Page: 89
Publisher Id: TOBEJ-12-75
DOI: 10.2174/1874120701812010075

Article History:

Received Date: 22/8/2018
Revision Received Date: 1/10/2018
Acceptance Date: 2/10/2018
Electronic publication date: 18/10/2018
Collection year: 2018

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© 2018 Mari Akiyama.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biomaterials, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan; Tel: +81 72 864 3056; Fax: +81 72 864 3156; E-mail:


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