RESEARCH ARTICLE
Characterization of Cells Interactions with Patterned Azopolymer-Based Materials using SEM, AFM and Video Microscopy
Régis Barillé1, Philippe Codron2, 3, Guillaume Mabilleau4, Florence Manero4, Romain Mallet4, Sonia Zielinska5, Ewelina Ortyl5, Joël Eyer6, Franck Letournel3, 6, *
Article Information
Identifiers and Pagination:
Year: 2018Volume: 12
First Page: 92
Last Page: 100
Publisher ID: TOBEJ-12-92
DOI: 10.2174/1874120701812010092
Article History:
Received Date: 02/08/2018Revision Received Date: 8/11/2018
Acceptance Date: 14/11/2018
Electronic publication date: 30/11/2018
Collection year: 2018
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Introduction:
Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.
Methods:
Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.
Results & Discussion:
We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).