RESEARCH ARTICLE
In Vitro Generation of Cartilage-Carrier-Constructs on Hydroxylapatite Ceramics with Different Surface Structures
Katharina Wiegandt1, Christiane Goepfert1, Teresa Richter1, Daniel Fritsch2, Rolf Janßen2, Ralf Pörtner*, 1
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 64
Last Page: 70
Publisher ID: TOBEJ-2-64
DOI: 10.2174/1874120700802010064
Article History:
Received Date: 19/9/2008Revision Received Date: 17/11/2008
Acceptance Date: 18/11/2008
Electronic publication date: 30/12/2008
Collection year: 2008
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Tissue engineering approaches for healing cartilage defects are partly limited by the inability to fix cartilage to bone during implantation. To overcome this problem, cartilage can be - already in vitro - generated on a ceramic carrier which serves as bone substitute. In this study, the influence of a hydroxylapatite carrier and its surface structure on the quality of tissue engineered cartilage was investigated. Application of the carrier reduced significantly biomechanical and biochemical properties of the generated tissue. In addition, slight changes in the quality of the formed matrix, in the adhesive strength between cartilage and biomaterial and in attachment and proliferation of a chondrocyte monolayer could be observed for commercial grade carriers, with respect to modified topographies obtained by smooth grinding/polishing. These first results demonstrated an influence of the carrier and its surface structure, but further research is needed for explaining the described effects and for optimization of cartilage-carrier-constructs.