The Open Biochemistry Journal




ISSN: 1874-091X ― Volume 13, 2019
RESEARCH ARTICLE

Short Exposures to an Extremely Low-Frequency Magnetic Field (ELF MF) Enhance Protein but not mRNA Alkaline Phosphatase Expression in Human Osteosarcoma Cells



Tania Rescigno, Anna Capasso, Bruno Bisceglia, Mario Felice Tecce*
Department of Pharmacy, University of Salerno, Fisciano (SA), Italy

Abstract

Background:

Among electromagnetic fields treatments used in orthopedics, extremely low-frequency magnetic fields (ELF MF) need more detailed information about the molecular mechanisms of their effects and exposure conditions.

Objective:

Evaluation of the effects of an ELF MF exposure system, recently introduced among current clinical treatments for fracture healing and other bone diseases, on Alkaline Phosphatase (ALP) activity and expression in a human osteosarcoma cell line (SaOS-2), as marker typically associated to osteogenesis and bone tissue regeneration.

Method:

Cells were exposed to the ELF MF physical stimulus (75 Hz, 1.5 mT) for 1h. Cell viability, enzymatic activity, protein and mRNA expression of alkaline phosphatase were then measured at different times after exposure (0, 4 and 24 h).

Results:

Data demonstrate that this signal is active on an osteogenic process already one hour after exposure. Treatment was, in fact, capable, even after an exposure shorter than those commonly used in clinical applications, to significantly up-regulate alkaline phosphatase enzymatic activity. This regulation is produced essentially through an increase of ALP protein level, without changes of its mRNA concentration, while assessed magnetic field did not affect cell growth and viability and did not produce temperature variations.

Conclusion:

Tested low-frequency magnetic field affects cellular ALP expression with a posttranslational mechanism, without the involvement of regulations at gene transcription and mRNA level. This molecular effect is likely produced even within treated tissues during therapies with this signal and may be implicated in the induction of observed effects in treated patients.



Article Information


Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 65
Last Page: 77
Publisher Id: TOBIOCJ-12-65
DOI: 10.2174/1874091X01812010065

Article History:

Received Date: 4/02/2018
Revision Received Date: 02/04/2018
Acceptance Date: 03/04/2018
Electronic publication date: 17/04/2018
Collection year: 2018

Article Metrics:

CrossRef Citations:
0

Total Statistics:

Full-Text HTML Views: 1944
Abstract HTML Views: 973
PDF Downloads: 485
ePub Downloads: 377
Total Views/Downloads: 3779

Unique Statistics:

Full-Text HTML Views: 960
Abstract HTML Views: 577
PDF Downloads: 337
ePub Downloads: 216
Total Views/Downloads: 2090
Geographical View

© 2018 Rescigno et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, I-84084 Fisciano (SA), Italy; Tel: +39 089 969772; Fax: +39 089 969602; E-mail: tecce@unisa.it


Endorsements



"We have always had a fruitful cooperation and long-term experience of publishing with Bentham Open."

"A number of our original papers have appeared in its different journals, as the Bentham Open Publishers provides sufficient research in the field of science and technologies (belonging to the pharmaceutical, biotechnology, biomedical industries etc). The staff of the Bentham Open always treat our papers very seriously and gently during the rutile paper reviewing process, so far we always felt their kindness and highly cooperative support. Today, Bentham Open is providing a great support to science and research, giving opportunities of high standard publishing as well as promoting and enhancing selected papers around the world through communication and exchange of global scientific education."


Kholmirzo T. Kholmurodov
Leading Scientist
FLNP (Frank Laboratory of Neutron Physics) JINR (Joint Institute of Nuclear Research) Moscow region, Russia


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2019 Bentham Open