RESEARCH ARTICLE
Micro-RNAs -106a and -362-3p in Peripheral Blood of Inflammatory Bowel Disease Patients
Ameneh Omidbakhsh1, Mohsen Saeedi2, Masoud Khoshnia3, Abdoljalal Marjani4, *, Safoura Hakimi1
Article Information
Identifiers and Pagination:
Year: 2018Volume: 12
First Page: 78
Last Page: 86
Publisher ID: TOBIOCJ-12-78
DOI: 10.2174/1874091X01812010078
Article History:
Received Date: 15/03/2018Revision Received Date: 01/06/2018
Acceptance Date: 04/06/2018
Electronic publication date: 29/06/2018
Collection year: 2018
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective:
MicroRNAs (miRNAs) can regulate various genes after binding to target mRNAs. Studies on Inflammatory Bowel Disease (IBD) in relation with miRNA are much less shown. The aim of the present study was to assess the expression patterns of microRNA 106a and microRNA 362-3p in peripheral blood samples of Inflammatory Bowel Disease (IBD) patients including Crohn’s Disease(CD) and Ulcerative Colitis (UC).
Methods:
This study consisted of 32 CD, 32 UC patients and 32 controls. The expression level of the micro-RNAs -106a and -362-3p was determined using reverse transcription and real-time RT-PCR.
Results:
Our findings showed that MiR-106a and miR-362-3p are expressed at significantly higher levels in the peripheral blood from patients with CD and UC compared to controls. MiR-106a and miR-362-3p expression are also different in the peripheral blood ofpatients regarding the activity score of the disease. There were significant differences of miR362-3p in active UC relative to inactive UC.
Conclusion:
Altogether our findings suggest that miR-106a and miR-363-3p can play an important role in the pathogenesis of IBD. The differences in expression of miR106a and miR362-3p in peripheral blood of the UC and CD patients in an active phase in comparison to inactive disease suggest that these miRNAs may be useful as potential biomarkers for diagnosis and monitoring the disease activity.
