RESEARCH ARTICLE
Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust
Abdollah Gholami1, Farideh Golbabaei2, *, Gholamheidar Teimori3, 4, Mojtaba Kianmehr5, Mehdi Yaseri6
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
First Page: 32
Last Page: 36
Publisher ID: TOBIOCJ-13-32
DOI: 10.2174/1874091X01913010032
Article History:
Received Date: 05/12/2018Revision Received Date: 05/03/2019
Acceptance Date: 10/03/2019
Electronic publication date: 30/04/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background and Objectives:
Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust.
Material and Methods:
In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99% silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The RAO et al. method was used to measure MDA.
Results:
The highest level of plasma MDA in group 1 in the 4th month was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5th group with the lowest concentration (P < 0.05).
Conclusion:
MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica.