The Open Biochemistry Journal

ISSN: 1874-091X ― Volume 14, 2020

Armadillo Repeat Containing 8α Binds to HRS and Promotes HRS Interaction with Ubiquitinated Proteins

Koji Tomaru1, Atsuhisa Ueda*, 1, Takeyuki Suzuki1, Nobuaki Kobayashi1, Jun Yang1, Masaki Yamamoto1, Mitsuhiro Takeno1, Takeshi Kaneko2, Yoshiaki Ishigatsubo1
1 Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan
2 Department of Pulmonary Medicine, Yokohama City University Medical Center, Yokohama 232-0024, Japan


Recently, we reported that a complex with an essential role in the degradation of Fructose-1,6-bisphosphatase in yeast is well conserved in mammalian cells; we named this mammalian complex C-terminal to the Lissencephaly type-1-like homology (CTLH) complex. Although the function of the CTLH complex remains unclear, here we used yeast two-hybrid screening to isolate Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) as a protein binding to a key component of CTLH complex, Armadillo repeat containing 8 (ARMc8) α. The association was confirmed by a yeast two-hybrid assay and a co-immunoprecipitation assay. The proline-rich domain of HRS was essential for the association. As demonstrated through immunofluorescence microscopy, ARMc8α co-localized with HRS. ARMc8α promoted the interaction of HRS with various ubiquitinated proteins through the ubiquitin-interacting motif. These findings suggest that HRS mediates protein endosomal trafficking partly through its interaction with ARMc8α.

Keywords: ARMc8α, FBPase, monoubiquitination, HRS, UIM.

Article Information

Identifiers and Pagination:

Year: 2010
Volume: 4
First Page: 1
Last Page: 8
Publisher Id: TOBIOCJ-4-1
DOI: 10.2174/1874091X01004010001

Article History:

Received Date: 30/10/2009
Revision Received Date: 30/11/2009
Acceptance Date: 10/12/2009
Electronic publication date: 13/1/2010
Collection year: 2010

© Tomaru et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: // which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Internal Medicine and Clinical Immunology Yokohama City University Graduate School of Medicine3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan; Tel: +81-45-787-2630; Fax: +81-45-786-3444; E-mail:

Track Your Manuscript:


"We have always had a fruitful cooperation and long-term experience of publishing with Bentham Open."

"A number of our original papers have appeared in its different journals, as the Bentham Open Publishers provides sufficient research in the field of science and technologies (belonging to the pharmaceutical, biotechnology, biomedical industries etc). The staff of the Bentham Open always treat our papers very seriously and gently during the rutile paper reviewing process, so far we always felt their kindness and highly cooperative support. Today, Bentham Open is providing a great support to science and research, giving opportunities of high standard publishing as well as promoting and enhancing selected papers around the world through communication and exchange of global scientific education."

Kholmirzo T. Kholmurodov
Leading Scientist
FLNP (Frank Laboratory of Neutron Physics) JINR (Joint Institute of Nuclear Research) Moscow region, Russia

Browse Contents

Webmaster Contact:
Copyright © 2020 Bentham Open