The Open Biochemistry Journal




ISSN: 1874-091X ― Volume 13, 2019

Endoplasmic Reticulum (ER) Stress Enhances Tip60 (A Histone Acetyltransferase) Binding to the Concanavalin A



Eun Jeoung Lee2, Sung Hwa Shin1, Sunghee Hyun2, Jaesun Chun3, Sang Sun Kang*, 1
1 Department of Biology Education, Chungbuk National University, Gaeshin-dong, Heungdok-gu, Cheongju, Chungbuk, 361-763, Republic of Korea.
2 Department of Pre-medicine, Eulji University School of Medicine, Daejeon 301-832, Republic of Korea
3 Department of Biology Education, Korea National University of Education, Chongwon, Chungbuk 363-791, Republic of Korea

Abstract

Herein, we report that the concanavalin A binding of Tip60 (a target of the human immunodeficiency virus type 1-encoded transactivator Tat interacting protein 60 KD; a histone acetyltransferase; HAT) is enhanced as the result of endoplasmic reticulum (ER) stress. The cell expression of Tip60 combined with site-directed mutagenesis analysis was used to identify the glutamine 324 residue as the lecithin binding (Concanavalin A; Con A) site. The Tip60 N324A mutant strain, which seems to be the Con A binding-deficient, was attenuated the protein-protein interactions with FE65 and its protein stability, but its ability of G0-G1 cell cycle arrest was not interrupted. Interestingly, both HAT activity and the nuclear localization of Tip60 N324A mutant were enhanced than those of Tip60 WT. Thus, our results indicate that the Con A binding deficient of Tip60 seems to be one of the most pivotal posttranslational modifications (such as N-glycosylation) for its functional regulation signal, which is generated in response to ER stress.

Keyword: Tip60, N-glycosylation, ER stress, HAT, Cell cycle.


Article Information


Identifiers and Pagination:

Year: 2012
Volume: 6
First Page: 1
Last Page: 10
Publisher Id: TOBIOCJ-6-1
DOI: 10.2174/1874091X01206010001

Article History:

Received Date: 10/10/2011
Revision Received Date: 6/2/2012
Acceptance Date: 14/2/2011
Electronic publication date: 9/3/2012
Collection year: 2012

Article Metrics:

CrossRef Citations:
0

Total Statistics:

Full-Text HTML Views: 2155
Abstract HTML Views: 1065
PDF Downloads: 268
Total Views/Downloads: 3488

Unique Statistics:

Full-Text HTML Views: 691
Abstract HTML Views: 540
PDF Downloads: 170
Total Views/Downloads: 1401
Geographical View

© Lee et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.


* Address correspondence to this author at the Department of Biology Education, Chungbuk National University, Gaeshin-dong, Heungdok-gu, Chungju, Chungbuk, 361-763, Republic of Korea; Tel: +82 43 261 3278; Fax: +82 43 271 0526; E- mail: jin95324@cbu.ac.kr


Endorsements



"We have always had a fruitful cooperation and long-term experience of publishing with Bentham Open."

"A number of our original papers have appeared in its different journals, as the Bentham Open Publishers provides sufficient research in the field of science and technologies (belonging to the pharmaceutical, biotechnology, biomedical industries etc). The staff of the Bentham Open always treat our papers very seriously and gently during the rutile paper reviewing process, so far we always felt their kindness and highly cooperative support. Today, Bentham Open is providing a great support to science and research, giving opportunities of high standard publishing as well as promoting and enhancing selected papers around the world through communication and exchange of global scientific education."


Kholmirzo T. Kholmurodov
Leading Scientist
FLNP (Frank Laboratory of Neutron Physics) JINR (Joint Institute of Nuclear Research) Moscow region, Russia


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2019 Bentham Open