Abstract HTML Views: 377 PDF Downloads: 204 Total Views/Downloads: 793
Abstract HTML Views: 241 PDF Downloads: 144 Total Views/Downloads: 517
In the present study Cx43 mRNA and protein were analyzed in germ cells of men with normal spermatogenesis
and in human testicular seminoma. In normal testis Cx43 mRNAs were basally located within seminiferous tubules and
expressed in the most basally located germ cells (spermatogonia, early spermatocytes, and pachytene spermatocytes) and
in Sertoli cells. Immunofluorescence analysis showed that Cx43 signal was mainly located in the basal compartment of
seminiferous tubules and was stage-dependent. Cx43 mRNAs were also detected in human testicular seminoma.
Transcripts were present within seminoma cells identified by PLAP staining. However, Cx43 protein exhibited an
intracytoplasmic accumulation, within an intracellular compartment distinct from the Golgi apparatus and was
undetectable at the plasma membrane level, suggesting post-translational rather than transcriptional abnormalities. This
aberrant intracytoplasmic accumulation of Cx43 is due neither to a dysfunction of the protein trafficking machinery nor to
a specific alteration of its major protein partner, ZO-1, since the tight junction associated protein was detected at the
plasma membrane level and did not colocalize with Cx43.