RESEARCH ARTICLE


The Role of MicroRNAs (miRNA 155, miRNA-146b) and Procalcitonin as Novel Markers for the Diagnosis of Spontaneous Bacterial Peritonitis



Dalia M. A. El-Hassib1, Dina M. Abo-elmatty2, Noha M. Mesbah2, Sherief Abd-Elsalam3, *, Shorouk A. Bastawisy2, Doaa E.4, Ayat S.E.M. Hassan5, Fawkia E. Zahran6, Reda S. Abdelghany7, Reham I. Siddik8, Asmaa R. Abdel-Hamed2
1 Department of Clinical and Chemical Pathology, Benha Faculty of Medicine, Benha University, Egypt
2 Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
3 Department of Tropical Medicine, Tanta University, Tanta, Egypt
4 Department of Biochemistry, Misr University for Science and Technology, Giza, Egypt
5 Department of Electron Microscopy, Theodor Bilharz Research Institute, Giza, Egypt
6 Department of Internal Medicine, Faculty of Medicine (Girls), Al -Azhar University, Cairo, Egypt
7 Department of Tropical Medicine, Ahmed Maher Teaching Hospital, Cairo, Egypt
8 Department of Internal Medicine, National Research Center, Cairo, Egypt

Article Information


Identifiers and Pagination:

Year: 2021
Volume: 11
First Page: 28
Last Page: 38
Publisher ID: TOBIOMJ-11-28
DOI: 10.2174/1875318302111010028

Article History:

Received Date: 11/9/2020
Revision Received Date: 21/12/2020
Acceptance Date: 25/1/2021
Electronic publication date: 19/04/2021
Collection year: 2021

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Creative Commons License
© 2021 El-Hassibet al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, Tanta University, El-Giash Street 31527, Tanta, Egypt; Tel: +2-01224608774, E-mail: sherif.abdelbaky@med.tanta.edu.ed


Abstract

Background:

MircoRNAs are endogenous, small non-coding RNA molecules that have been recognized as important modulators of gene expression. MicroRNA is considered one of the potential biomarkers of infection and inflammation. Our study aims to identify the potential role of miRNA-155, miRNA-146b, and Procalcitonin (PCT) in the early detection of spontaneous bacterial peritonitis in cirrhotic liver patients. miRNA-155 and 146b are molecular biomarkers , while procalcitonin is a serum marker in ascites patients complicated with Spontaneous Bacterial Peritonitis (SBP) .

Methods:

This study was conducted on 199 patients, 101 of them have ascites complicated with spontaneous bacterial peritonitis, and 98 patients without spontaneous bacterial peritonitis (control group). Ascitic fluid samples were collected from patients with SBP undergoing paracentesis at National Hepatology Institute in Egypt. MicroRNAs were determined in the serum using qPCR (quantitative polymerase chain reaction), while procalcitonin has been assessed in serum samples using ELISA (Enzyme-linked immune assay) technique.

Results:

Serum levels of miRNA-146b & miRNA-155 were significantly higher (p<0.001) in spontaneous bacterial peritonitis patients (79.2% and 97.0% respectively) than ascites patients (17.3% and 7.1%, respectively). Furthermore, the serum level of procalcitonin was significantly higher (p<0.001) in spontaneous bacterial peritonitis patients than that in ascites patients (68.3% and 27.6%, respectively).

Conclusion:

miRNA-155, miRNA-146b and procalcitonin can be used as early markers for the detection of SBP in hepatic patients with ascites.

Keywords: Ascites, Spontaneous bacterial peritonitis, miRNA-155, miRNA-146b, Procalcitonin, Syndrome.