REVIEW ARTICLE
Fixed Bed Reactors for the Cultivation of Mammalian Cells: Design, Performance and Scale-Up
R. Pörtner*, 1, O.B. Platas1, D. Fassnacht1, D. Nehring2, P. Czermak2, H. Märkl1
Article Information
Identifiers and Pagination:
Year: 2007Volume: 1
First Page: 41
Last Page: 46
Publisher ID: TOBIOTJ-1-41
DOI: 10.2174/1874070700701010041
Article History:
Received Date: 09/07/2007Revision Received Date: 17/08/2007
Acceptance Date: 17/08/2007
Electronic publication date: 28/8/2007
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Fixed-bed reactors have gained growing attention for the cultivation of mammalian cells. They allow for a low shear stress cultivation of adherent and non-adherent cells due to the immobilization of cells within macroporous carriers. Their potential has been demonstrated for many cell culture purposes. Some of the recent developments are presented in this review, including improved antibody production by hybridoma cells, high performance cultivation of a hepatoblastoma cell line and cultivation of cells for the production of retroviral vectors. Furthermore, criteria for the selection of process strategies and scale-up concepts are addressed.