REVIEW ARTICLE


Fixed Bed Reactors for the Cultivation of Mammalian Cells: Design, Performance and Scale-Up



R. Pörtner*, 1, O.B. Platas1, D. Fassnacht1, D. Nehring2, P. Czermak2, H. Märkl1
1 Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Germany
2 University of Applied Sciences Giessen-Friedberg, Institute of Biopharmaceutical Technology, Germany


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Creative Commons License
© 2007 Pörtner et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Germany; E-mail: poertner@tuhh.de


Abstract

Fixed-bed reactors have gained growing attention for the cultivation of mammalian cells. They allow for a low shear stress cultivation of adherent and non-adherent cells due to the immobilization of cells within macroporous carriers. Their potential has been demonstrated for many cell culture purposes. Some of the recent developments are presented in this review, including improved antibody production by hybridoma cells, high performance cultivation of a hepatoblastoma cell line and cultivation of cells for the production of retroviral vectors. Furthermore, criteria for the selection of process strategies and scale-up concepts are addressed.