REVIEW ARTICLE
A Simple Method for the Estimation of Protein Retention in Hydrophobic Interaction Chromatography Under Different Operation Conditions
Andrea Mahn*, 1, 2, M. Elena Lienqueo2, Juan A. Asenjo2
Article Information
Identifiers and Pagination:
Year: 2007Volume: 1
First Page: 9
Last Page: 13
Publisher ID: TOBIOTJ-1-9
DOI: 10.2174/1874070700701010009
Article History:
Received Date: 01/03/2007Acceptance Date: 17/04/2007
Electronic publication date: 25/5/2007
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in process design and scale-up.