REVIEW ARTICLE


A Simple Method for the Estimation of Protein Retention in Hydrophobic Interaction Chromatography Under Different Operation Conditions



Andrea Mahn*, 1, 2, M. Elena Lienqueo2, Juan A. Asenjo2
1 Department of Chemical Engineering, Universidad de Santiago de Chile, Av. Libertador Bernardo O’Higgins 3363, Estación Central, Santiago, Chile
2 Centre for Biochemical Engineering and Technology, University of Chile, Santiago, Chile


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Creative Commons License
© 2007 Mahn et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Chemical Engineering, Universidad de Santiago de Chile, Av. Libertador Bernardo O’Higgins 3363, Estación Central, Santiago, Chile; Tel: (56-2) 7181833; Fax: (56-2) 6817135; E-mail: amahn_2000@yahoo.es


Abstract

Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in process design and scale-up.

Keywords: Hydrophobic interaction chromatography, chromatographic conditions, dimensionless retention time.