RESEARCH ARTICLE
Multi-Fixed-Bed Bioreactor System Applied for Bioprocess Development of Immobilized Lactic Acid Bacteria
Rebecca Faschian*, Sonakshi De, Ralf Pörtner
Article Information
Identifiers and Pagination:
Year: 2016Volume: 10
First Page: 1
Last Page: 9
Publisher ID: TOBIOTJ-10-1
DOI: 10.2174/1874070701610010001
Article History:
Received Date: 21/5/2015Revision Received Date: 14/8/2015
Acceptance Date: 12/11/2015
Electronic publication date: 19/2/2016
Collection year: 2016
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
The multi-fixed-bed bioreactor “Multiferm” provides a downscaled system for the development of fixed-bed processes with immobilized microorganisms or cells. It consists of twelve single fixed-bed units that can be operated independently. Different bacterial strains, carriers used for immobilization, media or flow rates can be studied in one compact experimental set-up. Experiments can be run in parallel to allow for determination of experimental errors. In these studies several continuous cultivations were carried out with Lactobacillus delbrueckii ssp. bulgaricus using different dilution rates and culture durations as proof-of-concept for the Multiferm. Parameters relevant for process design such as reproducibility of simultaneously performed cultures, optimal dilution rate and long-term performance were determined by means of volume specific lactate and biomass productivity. First indications on a reduced growth rate of the cells due to immobilization were found.