Recombinant Production and Molecular Docking Studies of Casoplatelin, a Bioactive Peptide
Ragothaman M. Yennamalli1, 2, Pulkit Anupam Srivastava3, Sheena D. Sarswati3, Vijay Kumar Garlapati3, *
1 Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, Madurai-625021, India
2 Department of Bioinformatics, School of Chemical and Biotechnology,SASTRA University,Thanjavur-613401, Tamil Nadu, India
3 Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat -173234, Himachal Pradesh, India
Bioactive peptides from κ-casein have immense therapeutic potential as prophylactic formulations. Among these, casoplatelin is a κ-casein derived bioactive peptide with anti-thrombotic activities.
Herein, we report the production of casoplatelin in an E. coli expression system (using a pBAD vector) and show in silico modeling of its interactions.
A synthetic DNA construct encoding casoplatelin was designed with pepsin cleavage sites before and after the synthetic construct to allow the release of the peptide from the pro-peptide.
A novel recombinant approach was demonstrated for the production of casoplatelin, and anti-platelet aggregation activities of the product were confirmed. Also, casoplatelin structures were characterized in silico and then implemented to determine potential structural interactions with fibrinogen.
The present study showcases the recombinant approach for biopeptide production and its interaction with fibrinogen through in silico approach.
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* Address correspondence to this author at the Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat -173234, Himachal Pradesh, India; Tel: +91 1792 239288; Fax: +91 1792 245362; E-mail: firstname.lastname@example.org