Measurement of the Bio-Mechanical Properties of Two Different Feeder Layer Cells
S. Romanazzo1, 2, 3, K. Uesugi4, 5, 7, A. Taniguchi1, 2, *, G. Forte6, K. Morishima5, 7
1 Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
2 Cell-Materials Interaction Group, Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan
3 School of Chemistry, University of New South Wales (UNSW), Sydney, Australia
4 Graduate School of Mechanical Engineering, Department of Mechanical Engineering, Osaka University, Yamadaoka, Suita, Osaka, 565-0871, Japan
5 Department of Mechanical Systems Engineering, Ibaraki University, 4-12-1 Nakanarusawacho, Hitachi, Ibaraki, 316-8511, Japan
6 Center for Translational Medicine (CTM), International Clinical Research Center (ICRC) of St. Anne's University Hospital, Brno, Czech Republic
7 The Center for Advanced Medical Engineering and Informatics, Osaka University, Yamadaoka, Suita, Japan
We here present our findings on 2 types of feeder layers, one composed of mouse embryonic fibroblasts (MEF) and the second one of mouse skeletal myoblasts (C2Cl2) feeder cells.
The 2 feeder layers present a dramatic variance of intrinsic stiffness (142.68 ± 17.21 KPa and 45.78 ± 9.81 KPa, respectively).
Results and Conclusion:
This information could be used for a better understanding of cells and cell microenvironment mechano-physical characteristics that are influencing stem cell commitment, in order to develop a suitable engineered tissue for cardiac and skeletal muscle repair and a bio-actuator.
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* Address correspondence to this author at the Cell-Materials Interaction Group, Biomaterials Unit, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Japan; Tel: 0000-0001-7835-2522; E-mail: email@example.com