REVIEW ARTICLE
Exploitation of for the Heterologous Production of Cellulases and Hemicellulases
S.H. Rose, W.H. van Zyl*
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 167
Last Page: 175
Publisher ID: TOBIOTJ-2-167
DOI: 10.2174/1874070700802010167
Article History:
Received Date: 29/08/2007Revision Received Date: 04/04/2008
Acceptance Date: 04/04/2008
Electronic publication date: 27/5/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Filamentous fungi of the group are native soil saprophytic fungi. Industrial strains of this group have been extensively used for the production of plant degrading enzymes for the food and beverage, animal feed and paper-and-pulp industries. Recombinant DNA technology allows for the overproduction of these enzymes in copious amounts. The advantages and limitations of A. niger as recombinant host for enzyme production are briefly discussed. Specific attention is devoted to the overproduction of several cellulases and hemicellulases to high homogeneity in the protease-deficient strain A. niger D15. The size, temperature and pH optima of the heterologous enzymes were shown to be similar to that of their natively produced counter parts. The optimization of enzyme production in dilute sugar cane molasses, using a recombinant strain producing the xylanase II of Trichoderma reesei as example, was also demonstrated.