REVIEW ARTICLE
Enhancement of Extracellular Pullulanase Production by Raoultella planticola DSMZ 4617 Using Optimized Medium Based on Sago Starch
Siew Ling Hii1, #, Tau Chuan Ling2, Rosfarizan Mohamad1, 3, Arbakariya B. Ariff1, 3, *
Article Information
Identifiers and Pagination:
Year: 2009Volume: 3
First Page: 1
Last Page: 8
Publisher ID: TOBIOTJ-3-1
DOI: 10.2174/1874070700903010001
Article History:
Received Date: 22/09/2007Revision Received Date: 13/09/2008
Acceptance Date: 05/12/2008
Electronic publication date: 15/1/2009
Collection year: 2009
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC.