RESEARCH ARTICLE


Benzalkonium Chloride Sterilization of Nonwoven Fibrous Scaffolds for Astrocyte Culture



Robin Ng#, Jesse Singh Gurm, Shang-Tian Yang*
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus OH 43210, USA


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Creative Commons License
© 2009 Ng et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the 140 West 19th Avenue, Columbus OH 43210, USA; Tel: (614) 292-6611; E-mail: yang.15@osu.edu


Abstract

Tissue engineering is an emerging field in biomedicine, holding enormous promise for regenerative medicine. Scaffolds, within which cells proliferate, are a controlling factor in tissue engineering applications. Upon fabrication, tissue scaffolds must undergo appropriate sterilization to eliminate contaminants. Current sterilization methods are either costly, time consuming, or ineffective. In this study, a quaternary salt, benzalkonium chloride (BAC), was used as a chemical agent for sterilization of nonwoven polyethylene terephthalate (PET) fibers and polylactic acid nanofibers. Treating the PET scaffolds with 0.1% (w/v) BAC for only 2 minutes was effective to eliminate bacterial contaminants in the fibrous matrices. In addition, astrocyte cells were successfully cultured in the PET scaffolds following BAC sterilization, demonstrating the suitability of BAC as a sterilization agent. This chemical sterilization method is also mild and nonabrasive to nanostructured materials such as electrospun polylactic acid nanofibers.

Keywords: Astrocyte, benzalkonium chloride, nanofiber, polyethylene terephthalate, polylactic acid, sterilization, tissue scaffold.