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Screening of bovine rumen contents for heat-resistant and micro-aerophilic cellulose-degrading microorganisms
was performed on carboxymethyl cellulose (CMC) plates at 37 °C-45 °C, and many isolates were found to produce
reducing sugars under a static condition. Of those isolates, R8 and R15, which efficiently produced reducing sugars from
CMC at a high temperature, were further examined. Taxonomic analysis classified both strains into Bacillus licheniformis.
Comparison with B. licheniformis NBRC12200 and Trichoderma reesei NBRC31329 as a type strain under various conditions
revealed that R8 and R15 had filter paper- and CMC-degrading abilities, which are absent in the B. licheniformis
type strain, and that both isolates produced reducing sugars from CMC more efficiently than did T. reesei type strain at a
high temperature under a static condition. These results suggest that R8 and R15 have a strong cellulose-degrading ability
at a relatively high temperature under a micro-aerophilic condition.