RESEARCH ARTICLE
Evaluation of DNA Plasmid Storage Conditions
Manabu Murakami*
Article Information
Identifiers and Pagination:
Year: 2013Volume: 7
First Page: 10
Last Page: 14
Publisher ID: TOBIOTJ-7-10
DOI: 10.2174/1874070701307010010
Article History:
Received Date: 27/04/2013Revision Received Date: 14/05/2013
Acceptance Date: 16/05/2013
Electronic publication date: 12/7/2013
Collection year: 2013
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Several pBR DNA plasmid storage conditions were evaluated in this study, including the storage form (liquid or dried on 3M paper), the buffer used to dissolve the plasmid, and temperature. Storage in the liquid form resulted in increased colony formation compared to DNA dried on paper, which is a more convenient and often preferred method for shipping plasmids. The TE buffer was superior to autoclaved H2O for DNA storage. The most important factor for longterm storage was temperature, and storage at -20°C in TE buffer showed good results (ca. 107 cfu/mg plasmid DNA) for up to 270 days. In contrast, stored DNA plasmid in H2O or dried on What man 3M paper at 20°C, preferred for ordinary DNA shipment, resulted in deteriorated yields after 90 days. In conclusion, my results indicate that 20°C is acceptable for short-term storage (up to 18days), but TE buffer and -20°C should be used for longer-term storage.