RESEARCH ARTICLE


Analysis Fatty Acids Profile in Tabanus bivittatus Mats with Gas Chromatography-Mass Spectrometry



Wang Yanhua*, Wu Fuhua, Guo Zhaohan, Peng Mingxing, Xia Min, Pang Zhenling, Wang Xiaoli, Liang Zian, Zhang Naiqun
Life Science and Technology College, Nanyang Normal University, Henan Province, China


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Creative Commons License
© 2015 Yanhua et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the School of Life Science and Technology, Nanyang Normal University, Nanyang, Henan,473000, P.R. China; Tel: +86 18567179077; E-mail: wanga_yanhua@163.com


Abstract

Tabanus bivittatus Mats., a traditional Chinese medicine, is commonly used for cardiovascular disorders treatment including atherosclerosis. There have been only a few researches on its chemical components, and no detailed report has appeared on its fatty acids. To develop a simple and effective method for the extraction of total fatty acids from Tabanus bivittatus Mats., the Soxhlet extraction (SE) condition was optimized with response surface methodology. The fatty acid composition of the extract were determined by GC-MS with previous derivatization to fatty acid methyl esters (FAMEs). The major fatty acids in Tabanus bivittatus Mats. were oleic acid, palmitic acid, linoleic acid, palmitoleic acid, and stearic acid, and the unsaturated fatty acids occupy 63.9% of the total fatty acids.

Keywords: Fatty acid, gas chromatography, mass spectrometry, response surface methodology, Tabanus bivittatus Mats.