The Use of Single Base Extension (SBE) for the Assessment of European Mitochondrial DNA Haplogroups in Galician Population
Ignacio Rego1, Mercedes Fernandez-Moreno 1, Sara Relañ1, Joaquin Arenas2, Francisco J. Blanco*, 1
1 Osteoarticular and Aging Research Lab, Biomedical Center Research, Rheumatology Division, CH Universitario Juan Canalejo, Xubias 84, 15006, A Coruña, Spain;
2 Laboratorio Investigacion Enfermedades Mitocondriales, Hospital 12 de Octubre, Madrid, Spain
Abstract
Evidences are accumulating on the effects of the mtDNA variants on many complex traits due to the contribution of mtDNA to cellular physiology. More and more studies are being carried out in order to investigate the possible associations between these mtDNA variants or haplogroups and multifactorial complex diseases. Since the required sample size is high, these large-scale epidemiology studies require a suitable method to assign the mtDNA haplogroups in a rapid, accuracy and cost effective manner. In this study we present an assay, based on primer base extension, which permits the rapid genotyping of samples for the assessment of major European mtDNA haplogroups.
Keywords: mtDNA haplogroups, single base extension, galician population, PCR-RFLP.
Article Information
Article History:
Received Date: 14/11/2007
Acceptance Date: 15/12/2007
Electronic publication date: 25/1/2008
Collection year: 2008
Bentham Science Publishers Ltd
* at the Osteoarticular and Aging Research Laboratory, Biomedical Research Center, Complejo Hospitalario Universitario Juan Canalejo, 15006-A Coruña, Spain; Tel: 34-981-178272; Fax: 34-981-178273;
E-mail: fblagar@canalejo.org
Open Peer Review Details |
Manuscript submitted on 14-11-2007 |
Original Manuscript |
The Use of Single Base Extension (SBE) for the Assessment of European Mitochondrial DNA Haplogroups in Galician Population |