The Open Cardiovascular Medicine Journal

ISSN: 1874-1924 ― Volume 13, 2019

Cannabinoid Receptor 2 Deficiency in Haematopoietic cells Aggravates Early Atherosclerosis in LDL Receptor Deficient Mice

Dianne J.M Delsing1, *, Frank P Leijten1, Karin Arts1, Hans van Eenennaam1, Anja Garritsen1, Marion J.J Gijbels2, 3, Menno P.J de Winther2, Andrea van Elsas1
1 MSD, Department of Immune Therapeutics, Oss, The Netherlands
2 Department of Molecular Genetics, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
3 Department of Pathology, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands



The cannabinoid receptor 2 (CB2) has been implicated to play a role in various inflammatory processes. Since atherosclerosis is currently considered a chronic inflammatory disease, we studied the effect of haematopoietic CB2 deficiency on atherosclerosis development.

Methods and results:

To investigate the effect of CB2 deficiency in immune cells on atherogenesis in vivo, a bone marrow transplantation was performed in irradiated LDL receptor deficient mice (LDLr-/-), using CB2 deficient (CB2-/-) or wildtype (WT) donor mice. After 12 weeks on a high fat-high cholesterol diet, en face analysis showed that atherosclerosis in the aortic arch was significantly increased in CB2-/- transplanted animals (6.40 ± 3.21%) as compared to WT transplanted mice (3.85 ± 1.61%). Although the total lesion area in the aortic root was not significantly different between WT and CB2-/- transplanted mice (0.45 ± 0.13 mm2 and 0.51 ± 0.17 mm2, respectively), CB2-/- transplanted mice showed a significantly larger plaque area (0.13 ± 0.07 mm2) than WT transplanted mice (0.08 ± 0.05 mm2) in the aortic valve in which atherogenesis is in an earlier stage than in the other aortic valves.


Lack of endocannabinoid signaling via the CB2 receptor aggravates early atherosclerosis development in LDLr-/- mice, suggesting that CB2 specific activation may prevent the development of atherosclerosis.

Keywords: Atherosclerosis, cannabinoid receptor, CB2, LDLr-/- mice..

Article Information

Identifiers and Pagination:

Year: 2011
Volume: 5
First Page: 15
Last Page: 21
Publisher Id: TOCMJ-5-15
DOI: 10.2174/1874192401105010015

Article History:

Received Date: 6/12/2010
Revision Received Date: 27/12/2010
Acceptance Date: 30/12/2010
Electronic publication date: 15/2/2011
Collection year: 2011

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© Delsing et al.; Licensee Bentham Open.

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* Address correspondence to this author at the Department of Immune Therapeutics, Oss, The Netherlands; Tel: +31 412 66 3360; Fax: +31 412 66 2553; E-mail:


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