The Open Dentistry Journal

ISSN: 1874-2106 ― Volume 14, 2020

Comparison of Saliva Nitric Oxide between Chronic Kidney Disease Before and After Dialysis and with Control Group

Fatemeh Rezaei1, *, Reza Mohhamadi2
1 Department of Oral Medicine, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran



Chronic Kidney Disease (CKD) is a chronic progressive disorder and a major cause of death and disability in all countries. In the kidneys, Nitric Oxide (NO) has involved in several important cellular processes including glomerular and modular hemodynamics set-out, tubular - glomerular feedback reaction, renin releasing and extracellular fluid volume but NO can act as an inflammatory mediator and oxidative stress factor in high levels.


The aim of this study was to evaluate salivary levels of NO in patients with chronic kidney disease on dialysis compared to the healthy subjects and evaluate the effect of dialysis on the level of NO in saliva.

Materials & Methods:

In this case-control study, 30 hemodialysis patients and 30 healthy controls that were matched for age and sex were selected. Unstimulated saliva samples were collected from all subjects. In the patient’s group, half an hour before starting dialysis first sampling and two hours after the completion of dialysis second sampling were collected. NO concentration in the samples was measured by using the Griess method. For data analysis, SPSS software version 16, Mann Whitney-U and Wilcoxon test were used. The level of significance was considered 0.05.


Mann-Whitney U test showed that the average concentration of salivary NO in patients with CKD (pre-dialysis and after dialysis) was higher than in the control group. The average concentration of salivary NO in patients with CKD was reduced after hemodialysis.


Hemodialysis reduces salivary NO levels in CKD patients. It seems that hemodialysis has a role in decreasing the concentration of this inflammatory mediator and oxidative stress.

Keywords: Chronic kidney disease, Dialysis, Nitric Oxide, Saliva, Wilcoxon test, Glomerular Filtration Rate (GFR).

Article Information

Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 213
Last Page: 218
Publisher Id: TODENTJ-12-213
DOI: 10.2174/1874210601812010213

Article History:

Received Date: 26/12/2017
Revision Received Date: 21/02/2018
Acceptance Date: 28/02/2018
Electronic publication date: 28/03/2018
Collection year: 2018

© 2018 Rezaei and Mohhamadi.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Oral Medicine, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran; Tel: 98-918-8371081; Email:


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