The Open Dentistry Journal

ISSN: 1874-2106 ― Volume 14, 2020

Antibacterial Activity of Phytochemical Extracts and Endophytic Fungi of Carapa Guianensis Against Enterococcus Faecalis in Endodontic Infections An In Vitro Study

Maria do Carmo M. de Miranda1, Clarice M. Carvalho2, Fernando S.E.D.V. Faria2, Antonio M-S. Nobreza3, Thiago M. Pereira3, Keliin Pivatto3, Marcus V.C. da Costa4, Orlando A. Guedes3, Cyntia R. de Araújo Estrela3, Álvaro H. Borges3, *
1 Institute of Biodiversity and Biotechnology of the Legal Amazon, Federal University of Acre, Rio Branco, Acre, Brazil
2 Center for Biological and Nature Sciences, Federal University of Acre, Rio Branco, Acre, Brazil
3 Department of Oral Sciences, University of Cuiaba, Cuiaba, Mato Grosso, Brazil
4 Faculty of Medicine, Federal University of Goias, Goiânia, Goiás, Brazil



The objective of this study was to evaluate the antibacterial activity of phytochemical extracts and endophytic fungi of Carapa guianensis against Enterococcus faecalis. Carapa guianensis leaves and stems were collected to obtain phytochemical extracts and fungal metabolites and evaluated for in vitro antibacterial activity against E. faecalis using the disc diffusion method and dentin blocks with bacterial biofilm.


Thirty dentin blocks were prepared and contaminated for 60 days with E. faecalis. The specimens were randomly divided into 6 experimental groups according to the test solution used: G1 – hexane stem extract of Carapa guianensis; G2 – methanol stem extract of Carapa guianensis; G3 – methanol leaf extract of Carapa guianensis; G4 – ethyl acetate extract of the endophytic fungus Penicillium isolated from Carapa guianensis; G5 – negative control, with no addition of bacterial inoculum; G6 – positive control.


Bacterial growth was analyzed by spectrophotometry after 14 days of direct contact between the extracts and dentin blocks. The hexane-stem, methanol-stem, methanol-leaf, and ethyl-acetate endophytic fungus Penicillium extracts inhibited bacterial growth in 100% of the samples.


The present study demonstrated the antibacterial potential of phytochemical extracts and endophytic fungi of Carapa guianensis against E. faecalis.

Keywords:: Endodontics. Enterococcus faecalis. Phytochemicals, Carapa guianensis, Endophytic fungi, Penicillium.

Article Information

Identifiers and Pagination:

Year: 2019
Volume: 13
First Page: 249
Last Page: 254
Publisher Id: TODENTJ-13-249
DOI: 10.2174/1874210601913010249

Article History:

Received Date: 25/03/2019
Revision Received Date: 27/06/2019
Acceptance Date: 01/07/2019
Electronic publication date: 31/07/2019
Collection year: 2019

© 2019 De Miranda et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Oral Sciences, School of Dentistry, University of Cuiabá, Avenida Manoel José de Arruda, 3100, Jardim Europa, 78065-900, Cuiabá, MT, Brazil; Tel: +55 65 3363-1264;

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