The Open Dentistry Journal




ISSN: ― Volume ,
RESEARCH ARTICLE

The Affinity of Human Fetal Osteoblast to Laser-Modified Titanium Implant Fixtures



Lee Kian Khoo1, Sirichai Kiattavorncharoen1, Verasak Pairuchvej1, Nisanat Lakkhanachatpan2, Natthamet Wongsirichat1, Dutmanee Seriwatanachai2, *
1 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Rachathewee District, Bangkok, 10400, Thailand
2 Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Rachathewee District, Bangkok, 10400, Thailand

Abstract

Introduction:

Implant surface modification methods have recently involved laser treatment to achieve the desired implant surface characteristics. Meanwhile, surface modification could potentially introduce foreign elements to the implant surface during the manufacturing process.

Objectives:

The study aimed to investigate the surface chemistry and topography of commercially available laser-modified titanium implants, together with evaluating the cell morphology and cell adhesion of human fetal osteoblast (hFOB) seeded onto the same implants.

Method:

Six (6) samples of commercially available laser-modified titanium implants were investigated. These implants were manufactured by two different companies. Three (3) implants were made from commercially pure grade 4 Titanium (Brand X); and three were made from grade 5 Ti6Al4V (Brand Y). The surface topography of these implants was analyzed by scanning electron microscope (SEM) and the surface chemistry was evaluated with electron dispersive x-ray spectroscopy(EDS). Human fetal osteoblasts were seeded onto the implant fixtures to investigate the biocompatibility and adhesion.

Results & Discussion:

Brand X displayed dark areas under SEM while it was rarely found on brand Y. These dark areas were consistent with their organic matter. The hFOB cell experiments revealed cell adhesion with filopodia on Brand X samples which is consistent with cell maturation. The cells on Brand Y were morphologically round and lacked projections, one sample was devoid of any noticeable cells under SEM. Cell adhesion was observed early at 48 hrs in laser-irradiated titanium fixtures from both the brands.

Conclusion:

The presence of organic impurities in Brand X should not be overlooked because disruption of the osseointegration process may occur due to the rejection of the biomaterial in an in-vivo model. Nevertheless, there was insufficient evidence to link implant failure directly with carbon contaminated implant surfaces. Further studies to determine the toxicity of Vanadium from Ti6Al4V in an in-vivo environment should indicate the reason for different cell maturation.

Keywords: Bone formation, Biomaterial, Dental prosthesis, Osseointegration, Titanium, Affinity.


Article Information


Identifiers and Pagination:

Year: 2020
Volume: 14
First Page: 52
Last Page: 58
Publisher Id: TODENTJ-14-52
DOI: 10.2174/1874210602014010052

Article History:

Received Date: 05/08/2019
Revision Received Date: 20/12/2019
Acceptance Date: 02/01/2020
Electronic publication date: 18/02/2020
Collection year: 2020

© 2020 Khoo et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Rachathewee District, Bangkok, 10400, Thailand; Tel: +662007849;
E-mail: dutmanee.ser@mahidol.ac.th




Track Your Manuscript:


Endorsements



"Thank you for your magnificent and marvelous support throughout the publication stages. I would like to endorse my experience with you as a Junior Researcher and a recent graduate of the Dental school. Once again Thank you for your Great Help and Guidance throughout the stages of Publication. You guys are a great team and I am proud to be a Young Bentham author."


Asra Sabir Hussain
The University of Edinburgh Business School, UK

"My experience with Bentham Open was a valuable one because the quality of the services and analysis of my paper contributed in improving what we intended to convey to the readers."


José Ricardo Kina
Department of Surgery and Integrated Clinic,
Araçatuba School of Dentistry, São Paulo State University
Brazil

"The Publication Manager was very cooperative and replied my mails and guided me without any delays; however the reviewing process was too long."


Mahtab Memarpour
Prevention of Oral and Dental Disease Research Center,
Department of Pediatric Dentistry, School of Dentistry,
Shiraz University of Medical Sciences, Shiraz,
Iran


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2020 Bentham Open