The Open Dentistry Journal




ISSN: 1874-2106 ― Volume 13, 2019

Development of In Vitro Denture Biofilm Models for Halitosis Related Bacteria and their Application in Testing the Efficacy of Antimicrobial Agents



Tingxi Wu 1, Xuesong He 1, Hongyang Lu 2, David J Bradshaw 3, Alyson Axe 3, Zvi Loewy 4, Honghu Liu 1, Wenyuan Shi 1, Renate Lux 1, *
1 School of Dentistry, University of California Los Angeles, Los Angeles, USA
2 West China School of Stomatology, Sichuan University, Chengdu, China
3 GlaxoSmithKline, Family Oral Health, Weybridge, UK
4 Department of Pharmaceutical and Biomedical Sciences, Touro College of Pharmacy, New York, NY, USA

Abstract

Objective : Since dentures can serve as a reservoir for halitosis-causing oral bacteria, halitosis development is a concern for denture wearers. In this study, we surveyed the prevalence of four selected halitosis-related species (Fusobacterium nucleatum, Tannerella forsythia, Veillonella atypica and Klebsiella pneumoniae) in clinical denture plaque samples, and developed denture biofilm models for these species in vitro to facilitate assessment of antimicrobial treatment efficacy. Design : Denture plaque from ten healthy and ten denture stomatitis patients was screened for the presence of aforementioned four species by PCR. Biofilm formation by these halitosis-associated species on the surfaces of denture base resin (DBR) discs was evaluated by crystal violet staining and confocal laser scanning microscopy. The efficacy of denture cleanser treatment on these mono-species biofilms was evaluated by colony counting. Results : 80% of the subjects in the denture stomatitis group and 60% in the healthy group contained at least one of the targeted halitosis-related species in their denture plaque. All halitosis species tested were able to form biofilms on DBR disc surfaces to varying degrees. These in vitro mono-species resin biofilm models were used to evaluate the efficacy of denture cleansers, which exhibited differential efficacies. When forming biofilms on resin surfaces, the halitosis-related species displayed enhanced resistance to denture cleansers compared with their planktonic counterparts. Conclusion : The four selected halitosis-related bacterial species examined in this study are present on the majority of dentures. The mono-species biofilm models established on DBR discs for these species are an efficient screening tool for dental product evaluation.

Keywords: Antimicrobial treatment, biofilms, denture, halitosis, model.


Article Information


Identifiers and Pagination:

Year: 2015
Volume: 9
First Page: 125
Last Page: 131
Publisher Id: TODENTJ-9-125
DOI: 10.2174/1874210601509010125

Article History:

Received Date: 2/1/2015
Revision Received Date: 26/1/2015
Acceptance Date: 24/2/2015
Electronic publication date: 31 /3/2015
Collection year: 2015

Article Metrics:

CrossRef Citations:
0

Total Statistics:

Full-Text HTML Views: 2070
Abstract HTML Views: 1341
PDF Downloads: 331
Total Views/Downloads: 3742

Unique Statistics:

Full-Text HTML Views: 1076
Abstract HTML Views: 824
PDF Downloads: 243
Total Views/Downloads: 2143
Geographical View

© Wu et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.


* Address correspondence to this author at the Section of Oral Biology in the Division of Oral Biology and Medicine, School of Dentistry, University of California Los Angeles, 33-080 CHS, 10833 Le Conte Avenue, Los Angeles, CA, USA, 90095-1668: Tel: +13102065660; Fax: +13107947109; E-mail: rlux@dentistry.ucla.edu



Endorsements



"Thank you for your magnificent and marvelous support throughout the publication stages. I would like to endorse my experience with you as a Junior Researcher and a recent graduate of the Dental school. Once again Thank you for your Great Help and Guidance throughout the stages of Publication. You guys are a great team and I am proud to be a Young Bentham author."


Asra Sabir Hussain
The University of Edinburgh Business School, UK

"My experience with Bentham Open was a valuable one because the quality of the services and analysis of my paper contributed in improving what we intended to convey to the readers."


José Ricardo Kina
Department of Surgery and Integrated Clinic,
Araçatuba School of Dentistry, São Paulo State University
Brazil

"The Publication Manager was very cooperative and replied my mails and guided me without any delays; however the reviewing process was too long."


Mahtab Memarpour
Prevention of Oral and Dental Disease Research Center,
Department of Pediatric Dentistry, School of Dentistry,
Shiraz University of Medical Sciences, Shiraz,
Iran


Browse Contents



Webmaster Contact: info@benthamopen.net
Copyright © 2019 Bentham Open