RESEARCH ARTICLE
Skin Biopsy is Predictive of Outcome in Experimental Sepsis by Multidrug- Resistant Pseudomonas aeruginosa
Vassiliki Tziortzioti1, Haritini Petropoulou2, Thomas Tsaganos1, Aikaterini Spyridaki1, Maria Raftogiannis1, Evangelos J. Giamarellos-Bourboulis*, 1, Nicolaos G. Stavrianeas2
Article Information
Identifiers and Pagination:
Year: 2007Volume: 1
First Page: 1
Last Page: 5
Publisher ID: TODJ-1-1
DOI: 10.2174/1874372200701010001
Article History:
Received Date: 10/08/2007Revision Received Date: 06/09/2007
Acceptance Date: 14/09/2007
Electronic publication date: 26/10/2007
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
To evaluate whether histological findings of skin in sepsis by Pseudomonas aeruginosa could be a predictive factor of progression to death, histological alterations after challenge by one multidrug-resistant isolate were studied in 24 rabbits. Acute pyelonephritis was induced after ligation of the right ureter and injection of 108 CFU per kg of body weight into the renal pelvis. Biopsy samples of skin were taken on necropsy. Mean survival of animals after bacterial challenge was 5.23 days. Main histological findings of skin were inflammation and swelling of dermis; thickening of endothelium; presence of thrombi in vessels; necrobiotic changes of the hair follicles. Serum TNFα was negatively correlated to histology of dermis and follicles. Positive correlation was found between survival and swelling of dermis. It is concluded that prolongation of survival was accompanied by intense edema of the dermis. A punch skin biopsy might be a predictive factor of sepsis outcome.
