RESEARCH ARTICLE
SENCAR Mouse Skin Papillomas Induced by 6-Methylbenzo[a]pyrene Carry H-ras Codon 52 (CTALeu → CCAPro) and Codon 13 (GGCGly → GTCVal) Mutations
Dhrubajyoti Chakravarti*, Paula C. Mailander, Ercole L. Cavalieri, Eleanor G. Rogan
Eppley Institute for Research in Cancer and Allied Diseases, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA
Article Information
Identifiers and Pagination:
Year: 2007Volume: 1
First Page: 6
Last Page: 9
Publisher ID: TODJ-1-6
DOI: 10.2174/1874372200701010006
Article History:
Received Date: 16/11/2007Revision Received Date: 26/11/2007
Acceptance Date: 28/11/2007
Electronic publication date: 13/10/2007
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Organic smoke contains various methylated polycyclic aromatic hydrocarbon (PAH) carcinogens. Among these, 6-methylbenzo[a]pyrene (6-CH3BP) is a moderate carcinogen. In vitro, it can form both stable and depurinating adducts. Of these, only two depurinating adducts [BP-6-CH2-N7G and 6-CH2BP-(1&3)-N7G] have been detected in mouse skin. We examined seven 6-CH3BP-induced SENCAR mouse skin papillomas for the presence of oncogenic H-ras mutations. Two papillomas contained the codon 52 (CTALeu → CCAPro) and a third contained the codon 13 (GGCGly → GTCVal) mutation. These results suggest that the H-ras codon 52 mutation is an oncogenic mutation.
Keywords: H-ras mutations, 6-CH3BP, mouse, papilloma.
