The Open Medicinal Chemistry Journal

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Anethum graveolens L‎. Alleviates Sperm ‎Damage by Limiting Oxidative Stress ‎and Insulin ‎Resistance ‎in ‎Diabetic Rats ‎

Ebrahim Abbasi-Oshaghi1, 2, Iraj Khodadadi2, Fatemeh Mirzaei3, Mehrdad Ahmadi1, Heidar Tayebinia2, Mohammad Taghi Goodarzi4, *
1 Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Department of Anatomy, Hamadan University of Medical Sciences, Hamadan, Iran
4 Department of Biochemistry, Islamic Azad University, Shahrood Branch, Shahrood, Iran



It has been reported that diabetes is associated with sperm ‎damage and infertility.


The purpose of this experiment was to survey the effect of Anethum graveolens L. (Dill) powder on sperm profiles, oxidative stress, insulin resistance, and histological changes in male diabetic rats.


Male rats were randomly divided into 6 groups (n=7); group 1: normal rats, 2: normal rats + 100mg/kg Dill, 3: normal rats + 300mg/kg Dill, 4: diabetic rats, 5: diabetic rats + 100mg/kg Dill, and 6: diabetic rats + 300mg/kg Dill. After 2 months of treatments, the sperm profile, anti-oxidant activity, superoxide dismutase (SOD) activity, and malondialdehyde were measured. The histopathology of testis was evaluated. Hormonal changes and tumor necrosis factor-α (TNF-α) levels were measured by ELISA.


Total anti-oxidant and SOD activity in diabetic rats significantly decreased, while MDA concentration was significantly increased in the testis and pancreas of diabetic rats compared with control. However, the use of Dill significantly normalized these profiles. The treatment of diabetic rats with Dill changed the sperm parameters. The levels of testosterone, FSH, and LH in diabetic rats were significantly reduced, but the treatment with Dill did not alter the level of these hormones. Dill also significantly normalized testis morphological changes, insulin resistance, and inflammation.


The use of Dill normalized oxidative stress, inflammation, and insulin resistance in diabetic rats that correlated with sperm profile and testis histological changes. The treatment of diabetic rat models with Dill did not show harmful effects on sperm profiles.

Keywords: Dill, Diabetes, Insulin resistance, Herbal medicine, Anti-oxidant, ELISA, Anethum graveolens L.

Article Information

Identifiers and Pagination:

Year: 2020
Volume: 14
First Page: 35
Last Page: 44
Publisher Id: TOMCJ-14-35
DOI: 10.2174/1874104502014010035

Article History:

Received Date: 14/02/2020
Revision Received Date: 29/05/2020
Acceptance Date: 18/06/2020
Electronic publication date: 31/07/2020
Collection year: 2020

© 2020 Abbasi-Oshaghi et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biochemistry, Islamic Azad University, Shahrood Branch, Shahrood, Iran,

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