1 Laboratory of Experimental Neurophysiology,
Department of Neurology, Aeginition Hospital, Medical School, National and
Kapodistrian University of Athens, Greece
2 Mycology Research
Laboratory, Microbiology Department, Medical School, National and Kapodistrian
University of Athens, Greece
3 Department of Nursing, Faculty of
Human Movement and Quality of Life Sciences, University of Peloponnese, Sparta,
4 Department of Computer
Science and Biomedical Informatics, University of Thessaly, Greece
of Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School,
National and Kapodistrian University of Athens, Greece
Commensals of the human body can shift to a pathogenic phase when the host
immune system is impaired. This study aims to investigate the effect of seven
yeast and two bacterial commensals and opportunistic pathogens isolated from
blood and the female genital tract on the transepithelial electrical resistance
(TER) of human cervical epithelial cell cultures (HeLa). The pathogens
Candida tropicalis, C. parapsilosis,C. glabrata, C. krusei, C.
albicans and Saccharomyces cerevisiae, caused a significant decrease
in TER as compared to the controls; Lactobacillus spp caused a
significant increase in TER versus the controls and Escherichia coli
had no effect on the TER of the cell monolayers. The above data show that
Candida spp., S. cerevisiae and Lactobacillus spp. have a
non-selective effect on the TER of HeLa cell monolayers. These results are
consistent with the in vivo non-selective action of these microorganisms
on the various human mucosal epithelia.
Keywords: Candida, E. coli, HeLa cells, Lactobacillus, Saccharomyces, Transepithelial electrical resistance.
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* Address correspondence to this author at the Athens Medical School, Aeginition Hospital, Department of Biopathology and Clinical Microbiology, Vass. Sophias av. 72, 115 28 Athens, Greece; Tel/Fax: +30-2106004608;